Sherpa
A Macintosh-based Expert System for LC/MS & MS/MS Analysis
Sherpa is designed to be a robust, easy to use aide-de-camp in the correlation and
interpretation of LC/MS and MS/MS spectra to a known protein sequence or sequences.
Native files from Sciex APIIII, Sciex 100/300, and Finnigan SSQ/TSQ electrospray
mass spectrometers are currently supported. MS/MS files from other formats can be imported
as tab-delimited text files. Sherpa is licenced for distribution by the University of
Washington. A fully-featured demo and a new freeware lite version can be downloaded from
the download page.
Sherpa was developed by Alex Taylor and Richard Johnson in
Ken Walsh's Lab in
the Biochemistry Department at the
University of Washington in Seattle, WA.
SOME SHERPA FEATURES:
- EASY TO USE GRAPHICAL INTERFACE
- CALCULATION OF A PROTEIN'S MOLECULAR WEIGHT, DIGEST FRAGMENTS, AND DIGEST FRAGMENT CHARGE-STATE M/Z VALUES
- USER-DEFINABLE TERMINI, AMINO ACID RESIDUES, AND MODIFICATIONS
- SIMULTANEOUS ANALYSIS AGAINST MULTIPLE PROTEIN SEQUENCES
- COMPARISON OF TWO LC/MS FILES
- POSTTRANSLATIONAL MODIFCATION SEARCHES FOR GLYCOSYLATION AND PHOSPHORYLATION
DOCUMENTATION:
- Online Sherpa User Guide
- Downloadable version for viewing offline: Sherpa3.3.1DocHTML.sea.hqx (244 Kb).
Y2K COMPLIANCE:
- Sherpa is fully Y2K compliant.
FOR FURTHER REFERENCE:
View a poster presented at the 1996 ABRF conference in San Francisco describing the program.
An article describing Sherpa can be found in Rapid Communications in Mass Spectrometry :
Taylor, J.A., Walsh, K.A., Johnson, R.S. "Sherpa: A Macintosh-based expert
system for the interpretation of ESI LC/MS and MS/MS of protein digests".
Rapid Commun. Mass Spectrom. 10, 679-687 (1996).
Send Questions/Suggestions/Comments to taylora@hairyfatguy.com
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Last Updated May 1, 2004
J. Alex Taylor (taylora@hairyfatguy.com)